Composite

Part:BBa_K1300008:Design

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-19)


recA promoter + translational GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865


Design Notes

This construct was made for the purpose to test the recA promoter and ensure that it would be activated only when DNA damage occurs. Therefore, we had to ensure that this construct was designed with translational GFP (with RBS) so that GFP would be produced when the recA promoter is activated.

Source

Built from existing parts in the registry though BioBrick assembly. 

recA promoter [1] & translational GFP [2]

References